Thermal Stress–Induced Adaptive Responses in Pseudomonas aeruginosa: Phenotypic and Molecular Insights

Author(s): Wameedh Sabah Shukur1 , Teeba Azher Hammed2
Date: 09/10/2026
doi: https://doi.org/10.5281/zenodo.8425890

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Abstract

This study aimed to study the phenotypic adaptation and changes in medicine susceptibleness, and the 
transcriptomic changes in dispassionate P. aeruginosa isolates under warm stress condition.All the clinical 
isolates were subjected to higher temperature (42–45°C) and their growth parameters along with their 
ability to form biofilms and classification of antimicrobial resistance was examined.At the same time, 
RNA sequencing (RNA-seq) was utilized to discover explicit thermal guides for genes connected to 
verbalization of thermal strain: (Tata and others., 2016; Jozefczuk and others., 2010; Avican and others., 
2021).The results show that warm stress causes important phenotypic changes, including decreased 
biofilm formation and changed medicine sensitivities.Solids were observed in the genes that are associated 
with heat-shock proteins, stress-response managers, resentment-mixed pathway as well as metabolic and 
resistance pathway with transcriptomic analysis.(Avican and others., 2021; Behzadi and others., 2022; 
Redfern et al., 2021) To sum up, warm stress is a key factor in regulating the adjusting action of P. 
aeruginosa .By combining phenotypic and molecular studies, a more thorough understanding of the 
elasticity of the bacteria under unbiased conditions can be found, and more innovative strategies for 
controlling bacteria and treating disease can be developed.

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