Therapeutic Efficacy of Topical Sesame and Garlic Ointment Extract on Second-Degree Burn Wounds in Rabbits: A Comparative Histopathological Study

Author(s): Anhar. A.Khinfoor1, Sabreen .H.Hussein2, Zainab .S. Daaboul Al-Hassani3
Date: 08/10/2026
doi: https://doi.org/10.5281/zenodo.8425890

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Abstract

Background: The public health implications of burn injuries are substantial, and conventional burn 
treatments can result in prolonged healing time and negative side effects. Recently, there has been 
increased interest in using natural products and their active ingredients for use as alternative therapies for 
wound care. The purpose of this investigation was to determine the use of a new topical ointment 
composed of sesame oil and garlic extracts for treating second-degree burn injuries. Objective: The 
effects of sesame/garlic ointment extract on wound healing will be tested by measuring the area of the 
wound, the time required for the epithelial layer to heal, and the effects of the ointment on the appearance 
of histopathological changes in the tissue of the rabbits. 
Methods: A total of fifty New Zealand rabbits were randomly separated into two groups (n=25) as 
follows: Group A (the control group; received standard petroleum jelly) and Group B (the treatment group; 
received sesame and garlic ointment). Second-degree burn injuries were inflicted to the dorsum (back) of 
each rabbit. The sesame and garlic ointment was created using cold-pressed sesame oil containing sesamol 
and an alcoholic garlic extract (15% concentration containing allicin) blended with a base of petroleum 
jelly. The ointment was applied to the wounds in both groups twice daily for 14 days, and measurements 
of the area of each wound were recorded on days 0, 7, and 14. Tissue was also collected from the rabbits 
on days 7 and 14 for histopathological evaluations.

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