Analyzing Pseudomonas aeruginosa Isolates from Diwaniyah Hospitals, Iraq, for Beta-Lactamase Genetic and Behavioral Resistance Mechanisms Via Genetic Detection

Author(s): Saba Hameed Alshebani1 , Ameer Abed Kadhim Hamid2
Date: 08/10/2026
doi: https://doi.org/10.5281/zenodo.8425890

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Abstract

Hospital-related infections, particularly from intensive care units, are brought about by the bacteria 
Pseudomonas aeruginosa.  Because it is resistant to drugs and may exist in hospitals, it can be hard to 
kill off.  Because of this, it creates a significant risk and challenge in hospital situations.  The chemical 
and genetic traits of Pseudomonas aeruginosa, most notably its beta-lactamase synthesis and antibiotic 
resistance, have been part of several studies performed in the Al-Diwaniyah Province. One of the more 
crucial phases when developing antimicrobial tactics is identifying the biological indicators associated 
with antibiotic-resistant bacteria.  
While Pseudomonas aeruginosa had been isolated from a number of clinics in past studies, here remains 
not many assets available for its molecular characterization and resistance patterns.  Finding out more on 
a generalization of genes associated with resistance like GES, blaOXA-48, and SPM can help build 
approaches for managing bacterial infections.  Using traditional molecular techniques, this study sought 
to obtain and determine Pseudomonas aeruginosa from medical samples, investigate its capacity to 
resist drugs, and identify its presence of genes that create beta-lactamases. Findings: 15 out of 30 clinical 
specimens (50%) had Pseudomonas aeruginosa confirmed.  Burns (60%), wounds (26.6%), and urine 
(13.4%) had the greatest isolation rates.   

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